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This study was carried out on 25 bitches of different ages (2-4 years old), body weights and breeds.These bitches were brought to Theriogenology Clinic, Faculty of Veterinary Medicine, Cairo University with history of hemorrhagic discharge and difficult breeding.The maximum value of E17-B was in Alabai breed while the maximum value of P4 was in Pit bull breed [Table 1].3.2.Examination of the exfoliated vaginal epithelium The examined vaginal smear revealed presence of cornified epithelial cells and slight intermediate cells.

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Keywords: Vaginal hyperplasia, Histopathology, Surgical approach, Progestrone assay How to cite this URL: Galal SM, Fathi M, Ismail S T, El Belely M S, Mohamed FF.Surgical interference Two surgical approaches were done depending on the type of the hyperplastic mass; type 1 in which hyperplasia occurred only in the ventral portion of the vagina (the most common form), and type 2 in which hyperplasia comprised the whole vaginal wall (lateral, dorsal and ventral).The bitches were premedicated using atropine sulfate (0.04 mg/kg) followed by xylazine hydrochloride (2 mg/kg) as a muscle relaxant and the anaesthesia was induced by ketamine hydrochloride (10 mg/kg).Results: The current work revealed that the maximum value of estradiol 17-B was in Alabai breed while the maximum value of progesterone was in Pit bull breed.And Pit bull breed showed cornification and stratification of the vaginal mucosa with abundant eosinophilic cytoplasm and regular round nuclei.

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